Fig. 3

Over-represented Gene Ontologies and pathways in the secretome of C2 organoids. a Gene Ontology analysis of secreted proteins. The Search Tool for the Retrieval of INteracting Genes/proteins (STRING) database was used for prediction of protein subcellular localization. STRING confidence score represented the probability of finding functional modules that were selected if the significance (p-value) of GO enrichment was less than 0.01. Statistical significance was ascertained with a false discovery rate (FDR) threshold of <0.05. b PANTHER overrepresentation test. Proteins involved in metabolic process and regulation of extracellular activities were also major components of the C2 secretome. The table shows the most representative identified overrepresented categories of proteins involved in Parkinson disease, glycolysis and pentose phosphate pathways. Experiments were performed in duplicate. Data were considerate significant when p ≤ 0.05. c Network analysis was performed using Ingenuity Pathway Analysis software. The analysis shows the connection between the MIF, DDT (MIF-2) with AMPK embedded within the network. The network predicts the potential value and validity of the MIF-AMPK connection. d Summary of the modulation of AMPK activity on organoids through MIF