Fig. 2

Fbw7 overexpression promotes apoptosis in ABC-DLBCL. a Western blot showing increased Fbw7 expression in the ABC-DLBCL cell lines SU-DHL-2 and OCI-LY-3 after transfection with Fbw7 expression plasmid for 48 h. b Annexin-V–PE staining, followed by flow cytometry analysis to detect the percentage of apoptotic cells. In the indicated ABC-DLBCL cell lines, Fbw7 was upregulated by transfection (pcDNA3.1 vector was used as a negative control) for 24 h. Cell lines were cultured with Doxorubicin (1.0 μM) for 24 h to induce apoptosis before flow cytometry. Statistical significance was determined by a two-tailed, paired Student t test. **, P < 0.01; ***, P < 0.001. c Cell viability assays show that ABC-DLBCL cell lines were more sensitive to Doxorubicin after transfection with Fbw7 plasmid. ABC-DLBCL cells with 24 h exogenous Fbw7 expression were cultured with the indicated concentrations of Doxorubicin for 24 h before cell viability assays were performed. Statistical significance was determined by a two-tailed Student t test. ***, P < 0.001. Data are presented as mean ± SD from three independent experiments