Fig. 3

Fbw7 reduces the stability of Stat3. a western blotting of exogenous Stat3 in SUDHL-2 and OCI-LY-3 cells co-expressing Fbw7. b Fbw7 upregulation decreases endogenous Stat3 and especially pStat3^Tyr705 expression. c and d, Fbw7 decreases the stability of Stat3 and pStat3^Tyr705. Myc-Fbw7 was transfected into SUDHL-2 and OCI-LY-3 cells. After treating cells with cycloheximide (CHX; 10 mg/ml) for the indicated time intervals, Fbw7, Stat3 and pStat3^Tyr705 levels were examined by immunoblotting (top). Western blots were quantified via densitometry, and the mean ratios of the indicated proteins from three independent experiments are shown at the bottom of the figure. Data are shown as mean ± SD in the line graph for three independent experiments. Statistical significance was determined by a two-tailed unpaired Student t test. ***, P < 0.001