Fig. 7
From: Motility of glioblastoma cells is driven by netrin-1 induced gain of stemness
Invasive primary GBM cells increase Notch2 activation upon NTN1FH expression. a After 24Â h 3D Matrigel invasion spheroids consisting of GBM10 cells mixed with control U251, U251-NTN1FH or U251-NTN1(II)FH were fixed and used for immunofluorescence staining against cleaved Notch2 and HA. HA marks either full-length NTN1 (NTN1FH) or the central fragment of NTN1 (NTN1(II)FH). b Similarly as in (a) the invaded spheroids consisting of GM10 cells mixed with GFP positive U251MG cells were stained against cleaved Notch2. c Model of NTN1 induced invasion of GBM cells