Fig. 4

PI3K and COX-2 involved phenotype changes of HUVECs. a Phase contrast micrographs of capillary-like tubular structures on Matrigel. Magnification, x100. Graph bars showed the tube (left panel) and node (right panel) numbers of HUVECs treated with LY294002 or celecoxib in a co-culture system (*P < 0.05). b Apoptotic percentage of HUVECs with LY294002 treatment in the co-culture system as measured by TUNEL assay. Green: TUNEL-positive nuclei. Red: all nuclei. Bar graphs revealed the apoptotic percentage of HUVECs with LY294002 treatment after co-culture with CL1-5 cells (*P < 0.05). The data are presented as the mean ± SD. Experiments were performed in triplicate. c Immunoblot of cleaved Caspase 3 and PARP of HUVECs; α-tubulin was used as the loading control