Fig. 4

TMEM16A is a direct target of miR-381 in gastric cancer cells. a MiR-381 and its putative binding sequence in the wild-type and mutant 3′-UTR of TMEM16A. b Overexpression of miR-381 significantly decreased the luciferase activity that carried wild type (WT) but not mutant type (MUT) 3′-UTR of TMEM16A in gastric cancer cells. **P < 0.01. c, d Overexpression of miR-381 markedly suppressed the mRNA and protein levels of TMEM16A in gastric cancer cells. **P < 0.01. e Representative immunohistochemistry (IHC) staining of TMEM16A in high or low miR-381 expression gastric cancer tissues. f MiR-381 expression was inversely related to TMEM16A expression in gastric cancer tissues. **P < 0.01. g The correlation between miR-381/TMEM16A co-expression and the percentage of metastatic lymph nodes, P < 0.05. h Overall survival of gastric cancer patients. Patient groups were separated based on expression status of miR-381 and TMEM16A