Fig. 3

Identification of the expression of Notch pathway in RCC CSCs. The relative mRNA levels of notch1-3 (a and b), Jagged1/2 (c and d), DLL1, DLL3 and DLL4 (e and f) were detected using RT-PCR. (g and h) The up-regulation of notch1/2, Jagged1/2, DLL1/4 protein was validated by western blot analysis. ^* P < 0.05 VS. control; ^** P < 0.01 VS. control;^*** P < 0.001 VS. control; Control (Con):parental ACHN or Caki-1 cells