Fig. 4

The effects of notch signaling on stemness of RCC CSCs. The pharmacological (MRK-003) and endogenous (Numb) notch inhibitor suppressed the expression of notch1, notch2, Hes1 and Hey1 mRNAs (a, b, c and d), and stemness markers mRNAs (e and g) and proteins (f and h), decreased the self-renewal efficiency (i and j), invasion (k and l), migration (m and n), and sensitivity to cisplatin (o and p) and sorafenib (q and r) in RCC CSCs. ^* P < 0.05 VS. control; ^** P < 0.01 VS. control;^*** P < 0.001 VS. control; Control (Con): the RCC CSCs treated without inhibitor)