Fig. 4

PIG3 is required for microtubule growth in NSCLC cells. a Knock down of PIG3 by siRNA markedly inhibits the regrowth of microtubules from centrosomes in A549 cells. PIG3 and control siRNAs were transfected into A549 cells and 48 h post transfection the cells were treated with chilled medium + 1 μM nocodazole for an additional 1 h on the ice. Cells were fixed and stained with an anti-α-tubulin antibody. b The length of the microtubule emanating from the centrosomes was measured (n ≥ 50, ^** P <0.01). c Overexpressing PIG3 significantly increases microtubules growth rates in H1299 cells. d The length of the microtubule emanating from the centrosomes was measured (n ≥ 50, ^** P <0.01). e The localization of PIG3 in the cells detected by immunofluorescent staining