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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Sulfasalazine attenuates evading anticancer response of CD133-positive hepatocellular carcinoma cells

Fig. 2

Overexpression of CD133 inhibits ROS accumulation. a LCSC spheroids were reattached in a 384-well culture plate and stained with CM-H2DCFDA and CD133 to investigate the relationship between CD133 expression and intracellular ROS levels. b Expression of CD133 and ROS accumulation were detected after treatment with 200 μM H2O2 for 20 min in 2D culture condition. c Expression of CD133 in HCC cell lines was measured using flow cytometry and immunostaining analysis. d HCC cell lines were treated with 200 μM H2O2 for 20 min. ROS accumulation was analyzed by flow cytometry. e-f Huh7 cells were treated with the indicated concentrations of H2O2 and time. Whole cell lysates were then prepared and assessed by western blotting. Expression of CD133 was examined depend on the concentration (e) and treatment time (f) of H2O2. ß-actin was used as control

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