Fig. 2

Overexpression of CD133 inhibits ROS accumulation. a LCSC spheroids were reattached in a 384-well culture plate and stained with CM-H₂DCFDA and CD133 to investigate the relationship between CD133 expression and intracellular ROS levels. b Expression of CD133 and ROS accumulation were detected after treatment with 200 μM H₂O₂ for 20 min in 2D culture condition. c Expression of CD133 in HCC cell lines was measured using flow cytometry and immunostaining analysis. d HCC cell lines were treated with 200 μM H₂O₂ for 20 min. ROS accumulation was analyzed by flow cytometry. e-f Huh7 cells were treated with the indicated concentrations of H₂O₂ and time. Whole cell lysates were then prepared and assessed by western blotting. Expression of CD133 was examined depend on the concentration (e) and treatment time (f) of H₂O₂. ß-actin was used as control