Fig. 1

Curcumin inhibits gastric tumor cell growth in vitro and promotes apoptosis. a. RTCA was performed to determine the overall cell proliferation curve; n = 3. b. For cell viability determination, 6 × 10³SGC-7901 or BGC-823 cells were seeded into 96-well plates, treated with curcumin at 0,2.5,5,10,20,or 40 μg/mL) for 24 hand assayed based on MTT (Methods) For determination of proliferation, 2 × 10³ SGC-7901 or BGC-823 cells were seeded into five 96-well plates, treated with curcumin (10 μg/mL) for 0-5 days and the relative cell number was assayed using the CCK-8 kit (Method).; n = 5. c. 300 SGC-7901 or BGC-823 cells were cultured in 6-well plates, treated with10 μg/mL curcumin for 5 days, and stained with Giemsa dye for colony formation assessment (Methods); shown are representative dishes of colonies from the treatment groups, and the quantification of the colony number; n = 3. d. For apoptosis assessment, SGC and BGC cells were treated with 10 μg/mL curcumin for 24 h, prepared using the Annexin V kit (Methods), and analyzed by flow cytometry; shown are representative curves and the quantified data for the experimental groups; n = 3. e. Representative Western blot analysis of apoptosis-related proteins and signaling molecules of cell lysates from SGC or BGC cells treated with 10 μg/Ml curcumin, β-actin used as loading control; f. Mitochondrial membrane potential was determined from SGC or BGC cells treated with10 μg/mL curcumin and the cells prepared for analysis of the fluorescent membrane potential indicator, JC-1 (Methods); n = 3. g. Intracellular ROS was detected by using the fluorescent probe, DCFH-DA assay kit (Methods). SGC and BGC cells were treated with curcumin (10μg/ml) for 12 h and analyzed by FACs; n = 3. h. For mitochondrial superoxide production determination, SGC or BGC cells were treated with 10 μg/mL curcumin for 12 h,and were prepared for detection of MitoSOX fluorescence (Methods); n = 3. For B-D, F-H, data are presented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 compared to control or no DMSO group