Fig. 2

Chrysin inhibited cell proliferation and glycolysis in HCC cells. a, The chemical structure of chrysin. b, HCC cells were treated with indicated concentration of chrysin for indicated times, cell proliferation was measured as described in Material and Methods. The asterisk (*, p < 0.05) indicated a significant decrease of HCC cell proliferation after chrysin treatment. c-e, chrysin suppressed glycolysis in HCC-LM3 (top), SMMC-7721 (middle) and Bel-7402 (bottom) cells. HCC cells were treated with various concentrations of chrysin for 8 h and the cell lysates were subjected to SDS-PAGE to examine the change of indicated protein (left panels). Glucose consumption (middle panels) and lactate production (right panels) in cell culture medium were analyzed. The graph showed the data of at least three independent experiments expressed as means ± SD, the asterisks (*, p < 0.05, **, p < 0.01, ***, p < 0.001, Student’s t test) indicated significant inhibition of glucose consumption and lactate secretion after chrysin treatment