Fig. 4

Overexpression of HK-2 impaired the effect of chrysin on apoptosis and tumor glycolysis. a, activated caspase-3 and PARP in HCC cell with exogenous HK-2 expression. HCC cells were transfected with pORF-HK-2, 24 h later, the cells were treated with 60 μM chrysin, cell lysates were subjected to SDS-PAGE and probed with indicated protein. b and c, the effect of chrysin on glycolysis and apoptosis in HK-2 overexpressed HCC-LM3 cells. HCC-LM3 cell was transfected with pORF-HK-2 for 24 h and seeded in 6 well plates for 10 h, then exposed to 60 μM chrysin, tumor glycolysis (b) and apoptosis (c) was examined at 8 h and 24 h, respectively. b, The graph showed the data of at least three independent experiments expressed as means ± SD, the asterisks (*, p < 0.05, **, p < 0.01, ***, p < 0.001, Student’s t test) indicated significant difference between different groups. c, Representative FACS results of Annexin V-PI double staining were shown (left panels), and the graph (right panel) showed the data of at least three independent experiments expressed as means ± SD, the asterisks (***, p < 0.001, Student’s t test) indicated a significant difference