Fig. 5

SOX3 promotes Snail1 transcription via binding to the promoter region. a Sequence logo of SOX3 from JASPAR database. b Three putative SOX3-binding sites in Snail1 promoter region. c Truncation analysis of the Snail1 reporters. Five kinds of reporters, including upstream regions from bp −2340, −1267, −651 to bp +146, and upstream regions with site 3 deletion or site 3 substitution, were analyzed for their activity. (d, e) FLAG-tagged SOX3 variants (SOX3-ΔHMG and SOX3-ΔCTD) were followed by chromatin immunoprecipitation (ChIP) assay. Precipitated DNAs were quantified by qRT-PCR for promoter and non-promoter regions of Snail1 gene. The results are expressed as the mean ± SD of triplicate samples; * P < 0.05, **P < 0.01