Fig. 4

miR-20a-FBXL5/BTG3 signaling is responsible for iASPP-induced cell invasion and cisplatin resistance. a Venn diagram depicting 73 genes (including FBXL5 and BTG3) that were predicted potential targets of miR-20a, and were up-regulated by anti-20a in HeLa cells. b qPCR analysis of FBXL5 and BTG3 expression in CC cells after knockdown or overexpression of miR-20a. c Schematic representation of the 3’-UTR of FBXL5 or BTG3 with the predicted target site for miR-20a. d Expression of FBXL5 or BTG3 protein in CC cells after the transient knockdown or overexpression of miR-20a. e qPCR analysis of FBXL5 or BTG3 expression in CC cell lines and a normal cell line EM. f, g HeLa and SiHa cells were co-transfected with reporter plasmids containing the wild-type FBXL5 (f)/BTG3 (g) or a mutant FBXL5/BTG3 3’-UTR, together with a miR-20a mimic, miR-20a inhibitor or respective negative controls. The relative luciferase activity was assayed. h Immunoblot of FBXL5 (left) or BTG3 (right) protein expression in control or iASPP-silenced CC cells transfected with FBXL5 siRNA (FBXL5-Si), BTG3 siRNA (BTG3-Si) or control siRNA. i Representative images of invaded Hela cells (left) and relative invasion of control or iASPP-silenced CC cells after knockdown of FBXL5 or BTG3 (right). j Relative cell survival of control or iASPP-silenced CC cells after knockdown of FBXL5 or BTG3, upon exposure to cisplatin, as assessed by CCK8 assays. **P < 0.01