Fig. 2

Expression of AHNAK is significantly down-regulated in triple-negative breast cancer. a AHNAK expression level determined by qRT-PCR in two normal mammary epithelial cell lines, four luminal breast cancer cell lines and four basal-like breast cancer cell lines. β-actin was used as a control for normalization. The error bars represent the standard deviation (SD). * P < 0.05 and **P < 0.01. b Expression levels of AHNAK in 51 TNBC specimens and the corresponding adjacent normal tissues (Normal 1) (left) and 20 NTNBC specimens and the corresponding adjacent normal tissues (Normal 2) (right). Low levels of AHNAK correlate with poor prognosis. c DFS curves for 221 TNBC patients with high or low AHNAK levels. d OS curves for 221 TNBC patients with high or low AHNAK levels (right). e Representative immunohistochemistry images of AHNAK expression in two TNBC cases