Fig. 5

miR-21 induces the resistance of MFC cells to DDP in vitro and in vivo. a Cell viability assay of MFC cells treated with DDP after transfected with miR-21 mimics or miR-NC or without transfection (ctrl). b Colony formation assay of MFC cells transfected with miR-21 mimics or miR-NC or without transfection (up), and exposed to DDP (below). c Cell apoptosis assay of MFC cells treated with DDP after transfected with miR-21 mimics or miR-NC or without transfection (ctrl). The quantitative data are presented as the mean ± SD of triplicate experiments. (ns p > 0.05,*** p < 0.001). d and e The size and mean weight of tumors at the end of the experiment from mice inoculated with MFC cells transfected with miR-21 mimics or miR-NC. DDP or PBS (ctrl) treatment was initiated 10 days after subcutaneous injections of tumor cells. (n = 5, ns p > 0.05, ** p < 0.01)