Fig. 2

TCTP promotes GBC cell migration and invasion. a Western blot analysis of endogenous TCTP expression in six GBC cell lines. β-Actin was used as a loading control. b Analysis of TCTP expression in control and TCTP knock-down GBC cells. c-e Wound-healing, migration and invasion assays were used to compare GBC cells transfected with either scramble or TCTP shRNA. Cell motility, migration and invasion were quantified by setting the observations in the scramble controls as 100%. Each experiment was independently performed three times, and the graphed data represent the mean ± SD. **, p < 0.01