Fig. 6

miR-584-5p inhibits tumorigenicity in vivo. a, b. Photographs of tumors obtained from the different groups of nude mice transfected with miR-584-5p-mimics and miR-584-5p-inhibitor, respectively. c, d. Growth curve of tumor volumes were calculated. Date are shown as mean ± SD. Tumor volumes between miR-584-5p-mimics or miR-584-5p-inhibitor and corresponding control group was significantly different at p < 0.001 by two-way ANOVA. e. Tumor weight were calculated. Date are shown as mean ± SD. f, g, h, i. The expression levels of miR-584-5p and WWP1 protein in the implanted tumors that were transfected with miR-584-5p-mimics and miR-584-5p-inhibitor were explored by miRNA RT–PCR,qRT-PCR,Western blot and immunohistochemistry staining. j, k. Immunohistochemical staining against Ki-67 and TUNEL assay were used to determine the effects of miR-584-5p expression alteration on cell proliferation and apoptosis in the samples collected from nude mice. Scale bar is 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001. The data expressed as the mean ± SD