Fig. 2

Detection of HSF1 expression in different pancreatic cancer cell lines and HSF1-siRNA screening verification. a and b Western blot (a) and RT-PCR (b) were used to detect the expression levels of HSF1 in four pancreatic cancer cell lines (ASPC-1, BXPC -3, CFPAC-1 and PANC-1). c and d Detection of HSF1 expression in PANC-1 cells after transfection with HSF1-siRNA#1 and HSF1-siRNA#2 by Western blot (c) and RT-PCR (d). Differences were analyzed by paired t-test and data represent the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001