Table 1 Primers for real-time RT-PCR
From: Angiotensin II type 2 receptor promotes apoptosis and inhibits angiogenesis in bladder cancer
Gene | Sense | Sequence | Product size(bp) |
|---|---|---|---|
Mas | Forward Reverse | 5′- GCCTGTCAGTCCTTTACCCC-3′ 5′-CACAGAAGGGCACAGACCAA-3′ | 79 |
AT1R | Forward Reverse | 5′-CCGCATTTAACTGCTCACACA -3′ 5′-ATCATGTAGTAGAGAACAGGAATTGCTT -3′ | 213 |
AT2R | Forward Reverse | 5′-CGGAATTCATGAGCTGCGTTAATCC -3′ 5′-AACTGCAGTTAAGACACAAAGGTCTCCA -3′ | 165 |
Flt-1 | Forward Reverse | 5′- TCATGCTAATGGTGTCCCCG-3′ 5′-GTGCTGCTTCCTGGTCCTAAA-3′ | 99 |
Flk-1 | Forward Reverse | 5′-CAAGTGGCTAAGGGCATGGA -3′ 5′-ATTTCAAAGGGAGGCGAGCA-3′ | 181 |
VEGF | Forward Reverse | 5′-AGGCCAGCACATAGGAGAGA-3′ 5′- TACCGGGATTTCTTGCGCTT-3′ | 141 |
GAPDH | Forward Reverse | 5′-ACGGATTTGGTCGTATTGGG-3′ 5′-CGCTCCTGGAAGATGGTGAT-3′ | 214 |