Fig. 4

AR promotes the anti-metastatic effect of ERβ1. a Wound-healing assay for the cell migration of ERβ1-expressing MDA-MB-231 and Hs578T cells with or without DHT (10nM) treatment. The percentage of wound healing was calculated. b Representative images of transwell assays for the invasion of MDA-MB-231 and Hs578T cells treated with or without DHT. c Western blot analysis of ZEB1, ERβ1, E-cadherin and N-cadherin protein levels in ERβ1-expressing MDA-MB-231 and Hs578T cells after treatment with or without DHT. d qRT-PCR detection of ERβ1 mRNA in ERβ1-expressing MDA-MB-231 and Hs578T cells with or without DHT treatment. *p < 0.05