Fig. 2From: Ibrutinib, a Bruton’s tyrosine kinase inhibitor, exhibits antitumoral activity and induces autophagy in glioblastomaIbrutinib suppresses cell migration and induces apoptosis in GBM cells. (a) The migratory ability of LN229 and U87 cells was evaluated in a wound healing assay with cells treated with various concentrations of ibrutinib for 24 h. (b) The results of trans-well assay with LN229 and U87 cells treated with different concentrations of ibrutinib for 24 h. Statistical analyses of the migrated cells are shown on the right; **p < 0.01. (c) The percentage of apoptotic cells in LN229 and U87 cell population treated with increasing concentrations of ibrutinib, as detected by flow cytometry with annexin V-PI staining. Data are shown as the mean ± SD and are from three independent experiments; *p < 0.05, **p < 0.01. (d) The expression of apoptosis-associated proteins cleaved caspase 9, cleaved caspase 3, and Bcl-xL were detected by western blotting following the treatment of cells with increasing concentrations of ibrutinib for 48 hBack to article page