Fig. 3

Overexpression of RERG inhibited cell proliferation, colony formation, migration and invasion. a Growth curves of RERG-transfected and empty-vector-transfected NPC cells (HK1, C666-1, HK1_EBV). Data are means ± SD (n = 5). The insets show protein levels of RERG in each cell line transfected with empty and RERG vector. b ICC staining of HK1 stable cells against PCNA (magnification, ×200). PCNA-positive percentages in cultured HK1-RERG cells and HK1-empty cells (control) are presented as means ± SD (n = 4). c Colony formation assays. Cell colonies were stained with Giemsa. Quantitative analyses of colony formation efficiency show the means ± SD (n = 3) of at least 2 independent experiments. d Migration assay. Cells were seeded in a migration chamber and cultured for 24 h. Migratory cells were stained, photographed under a microscope and quantitatively analyzed. e Invasion assay. Cells were seeded in an upper chamber and cultured for 24 h. Invasive cells were stained, photographed under a microscope and quantitatively analyzed. Data are shown as means ± SD (n = 3) of at least 2 independent experiments. *: P < 0.05, **: P < 0.01, ***: P < 0.001 by Student’s t-test