Fig. 6
Effect of PKC inhibitors on PMA-induced cell differentiation. Kasumi-2 cells were treated with 10−8 M PMA in the presence or the absence of the Gö 6983 or the GF 109203X PKC inhibitor for 4 days, and the expression of SERCA3 and SERCA2, as well as of CD20, TdT and RAG-1 proteins was detected by Western blotting as described in Methods. Equal loading was controlled by Ponceau red staining. When compared to untreated cells (lane 1), PMA treatment alone (lane 2) led to the induction of SERCA3 and CD20 expression and the down-regulation of TdT and RAG-1 proteins. Gö 6983 or GF 109203X abolished the effects of PMA (lanes 3 and 4). When applied alone, Gö 6983 or GF 109203X had no significant effect of the cells (lanes 5 and 6)