Fig. 6
PHF21B suppresses the repressors of Wnt/β-catenin signaling pathway. a-b Real-time PCR analysis of SFRP1(a) and SFRP2 (b) expression in the indicated cells. c-d ELISA analysis of SFRP1(c) and SFRP2 (d) expression in the indicated cells. e-f Left panel: Luciferase activity assays in PC-3 and C4-2B cells showed trans-inactivation of the SFRP1(e) and SFRP2(f) promoters by PHF21B overexpression and trans-activation by PHF21B silencing. Right panel: ChIP enrichment assay confirmed that Flag-tagged PHF21B binds to the promoter of SFRP1 (e) and SFRP2 (f); IgG was used as a negative control. Schematic illustration of the indicated nucleotide regions of the SFRP1 and SFRP2 promoters. H3K27Ac enrichment, indicating high transcription activity, is observed in the promoter elements according to Genome Browser Gateway website. g Luciferase-reported TCF/LEF transcriptional activity in the indicated cells. h Histograms showing the mean number of spheres formed by the indicated cells. Each bar represents the mean ± SD of 3 independent experiments.*P < 0.05