Fig. 5

MiR-152-3p was involved in the regulation of NF2expression.mRNA expression of DNMT1, NF2, and miR-152-3p after transfection with miR-152-3p mimics, detected by RT-PCR in U251 cells (a). Protein expression of DNMT1 and NF2 was detected by Western blot after transfection with miR-152-3p mimics (b). The expression of DNMT1 and NF2in U251 cells was detected by immunofluorescence (c). After miR-152-3p transfection, the methylation status of NF2 in U251 cells was determined with MSP (d) and bisulfite Sanger sequencing (e). **, P < 0.01 compared with control groups treated with NC mimics