Fig. 7

DNMT1 was involved in apoptosis and invasion of U251 cells. The viability of U251 cells was determined at 24, 48 and 72 h after DNMT1 knockdown (a). At 48 h after DNMT1 knockdown, cell apoptosis was evaluated by flow cytometry (b) as well as Hoechst staining (c), and cell proliferation was detected with Edu (d); cell invasive activity was detected by transwell (e). *, P < 0.05, **, P < 0.01 compared with control groups