Fig. 8
From: Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis
NAC treatment affects EMT markers expression and cell proliferation in H460 cells (a). Real-time PCR analysis of E-Cadherin, Vimentin, Twist and Slug mRNAs expression were performed on total RNA extracted from H460shRNA, H460shFHC and H460shFHC cells treated with 5 mM NAC for 2 h. Final results represent mean ± SD of three independent experiments. Statistical significance was evaluated by Student t-test (*, p < 0.05, **, p < 0.01). b Proliferation rate of H460shRNA and H460shFHC cells with NAC (10 mM) was assessed by cell count at 24, 48 and 72 h. Final results represent mean ± SD of three independent experiments each performed in triplicate. Statistical significance was evaluated by Two-Way ANOVA (Sidak’s) (*, p < 0.05)