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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Wogonoside inhibits invasion and migration through suppressing TRAF2/4 expression in breast cancer

Fig. 6

Wogonoside inhibits TNF-α + TGF-β1-induced migration, adhesion and invasion in MCF7 cells in vitro. MCF7 cells were exposed to different concentrations of TNF-α, TGF-β1 and wogonoside for 48 h. a An MTT assay showed that TNF-α, TGF-β1 and wogonoside had no effect on cell viability. b Morphological changes in MCF7 cells were observed under an inverted lightmicroscope (400×). c 100 μl cell suspension (2 × 105 cells/ml) was added to the 96 wells which are pre-coated with matrigel. After incubating for 60 min, adherent cells were determined by MTT assay. d-e A monolayer of cells was scraped with a pipette tip and then treated with TNF-α, TGF-β1 and wogonoside. The migrating cells were assessed with a microscope equipped with a camera (image magnification: 100×). f-g The invasive ability was evaluated by a matrigel-coated transwell invasion assay (image magnification: 200×). h The expression of E-cadherin, MMP-9, MMP-2, CD44v6, and vimentin proteins in the cells were analyzed by western blot using specific antibodies. i E-cadherin, MMP-9, vimentin and Twist1 mRNAs were measured with real-time PCR. GAPDH was used as the internal control. The relative levels were calculated as the ratio of the relative biomarker mRNA to GAPDH mRNA. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group

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