Fig. 6
CCN2 binding with LRP6 occurs in a HSPGs-dependent Manner. a Dose-dependent promotion of adhesion of HCC cells was determined by incubation of MHCC97H cells in 96-well plates that had been pre-coated with 0–3 μg/ml recombinant human CCN2. b MHCC97H–LRP6-Sh2 exhibited decreased cell adhesion to CCN2 compared to untreated MHCC97H cells, and destruction of HSPGs with heparinase or inhibition of HSPG sulfation with NaClO3 exhibited almost complete inhibition effect for MHCC97H adhesion to CCN2. c Adhesion of HCC cells was determined following co-incubation of CCN2 with heparin. d LMWH inhibited the interaction between CCN2 and LRP6