Fig. 2

Sophoridine inhibits pancreatic cancer cell growth in both dose-dependent and time-dependent manners. a-c Two pancreatic cancer cells and one normal pancreatic ductal epithelial cell were assayed for cell viability after exposure to Sophoridine concentrations that ranged from 0 μM to 100 μM; cells treated with vehicle were set as controls. All the cells were cultured for 24 h, 48 h and 72 h. d At a fixed dose (20 μM), Sophoridine inhibited the viability of Pancreatic cancer cells and one normal pancreatic ductal epithelial cell in a time-dependent manner (24 h, 48 h, or 72 h). e Colony formation assays. Miapaca-2 and PANC-1 cells were treated with indicated doses of Sophoridine. The clones were visualized by crystal violet staining. f In the colony formation assay, the formed clones were counted manually for each group of cells. *, P < 0.05; **, P < 0.01