Fig. 6

ICG-001 mediates the association of β-catenin with CBP, P300, E-cadherin and N-cadherin. a and b Western blotting analysis of Wnt related proteins from total proteins and extracted nuclear and cytoplasmic proteins. c Confocal analysis of the expression and distribution of β-catenin in MGC-803 with or without ICG-001. d Coimmunoprecipitation of MGC-803 cells, performed as described in Materials and Methods, using 25 μM ICG-001. Anti-β-catenin, anti-CBP, anti-P300, anti-N-cadherin or anti-E-cadherin antibodies were used for the immunoprecipitation