Fig. 1

Upregulation of NUSAP1 expression in astrocytoma cell lines and tissues. a Based on data obtained from the Oncomine database, the expression of NUSAP1 was markedly upregulated in anaplastic astrocytoma (n = 19) and glioblastoma (n = 81) compared with normal brain tissues (n = 23). Error bars represent the mean ± SD values (**P < 0.05). b NUSAP1 expression was upregulated in grade IV astrocytoma (n = 156), followed by grade III astrocytoma (n = 131) and grade II astrocytoma (n = 63), according to The Cancer Genome Atlas (TCGA) database. Error bars represent the mean values determined using the Tukey test (**P < 0.05). c and d Real-time RT-PCR and western blotting analysis of NUSAP1 expression in astrocytoma tissue and brain tissue. The average NUSAP1 mRNA expression was normalized to the expression of GAPDH. GAPDH was used as a loading control. Error bars represented the mean ± SD values of three independent experiments (*P < 0.05). e and f Expression of NUSAP1 was upregulated in all seven astrocytoma cell lines compared with primary normal human astrocytes (NHAs), as confirmed by RT-PCR and western blotting analyses. GAPDH was used as a loading control. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05)