Fig. 2

SFN content in primed MSCs and control of their viability. a Determination of the SFN content of primed MSCs. MSCs were incubated for 1 h at 37 °C with SFN (20 μM or 100 μM). After washing with HBSS, the SFN content of the cell pellet was determined by HPLC. b Determination of the viability of SFN-primed MSCs. MSCs were incubated for 1 h at 37 °C with or without SFN (20 μM or 100 μM). After washing with HBSS, cells were seeded in a 96-well plate. Cell survival was estimated after 24 h, with the CyQUANT® cell proliferation assay kit. The results obtained for unprimed MSCs were considered to correspond to 100% survival. Data are expressed as the mean of three independent experiments ± SEM. Asterisks (*) indicate significant differences from unprimed MSCs (p < 0.05)