Fig. 6
From: Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells
CA impairs autophagic flux accumulating autolysosomes (AL) and aberrant autophagic vesicles/lipid droplets (AV/LDs). a Representative TEM images of SKBR-3 and BT474 cells untreated (Ctr), CA-treated (+CA), Tz and CA + Tz treated for 7d. Four major categories of structures could be distinguished by morphological criteria in CA-treated cells: multivesicular bodies (MVBs), autolysosomes (AL) autophagic vesicle/lipid droplets (AV/LDs) and lipid droplets (LDs). Nuclei (N), Golgi (G), mitochondria (mit). Bar = 700 nm. b-c Histograms showing the number and diameter of each endocytic/autophagic structures upon different treatments in SKBR-3 cells. For this analysis, 10 whole cells were scored and measured for each category of structures with iTEM imaging software. Note that in CA-treated cells the most represented category is AV/LDs, followed by AL and MVBs