Fig. 4

miR-143 and miR-107 target MSI-2 and inhibit CC cell proliferation and invasion. a, Predicted miR-143 and miR-107 binding sites in the 3′-UTR of MSI-2. b, miR-143 and miR-107 expression in normal cell line H8 and CC cell lines (HeLa and SiHa). c and d, Luciferase activities of reporter construct containing the wild-type (WT) or mutant (MU) 3′-UTR of MSI-2 in HeLa (c) and SiHa (d) cells transfected as indicated. e and f, Western blot analysis of HeLa (e) and SiHa (f) cells transfected as indicated. g, h and i, Quantification of invasion (g), proliferation (h) and sphere formation (i) of HeLa cells transfected as indicated. ^* P < 0.05