Fig. 3
From: TAGLN2 is a candidate prognostic biomarker promoting tumorigenesis in human gliomas
TAGLN2 knockdown decreases invasive ability of glioma cells. a Western blot of lysates (20 μg) from U87MG and U251 cells transfected with TAGLN2 and control siRNAs incubated with TAGLN2 antibody. GAPDH was used as a loading control. b Representative images of invaded spheroids in 3D invasion assay for U87MG, U251 and GBM#P3 cells transfected with TAGLN2 and control siRNAs evaluated at 48 h and 96 h are shown. Scale bar = 200 mm. c The area covered by invading cells quantitated after 96 h. NT: no treatment; Control: non-silencing siRNA; si-TAGLN2: siRNAs targeting TAGLN2