Fig. 6
From: TAGLN2 is a candidate prognostic biomarker promoting tumorigenesis in human gliomas
TGFβ stimulates TAGLN2 protein expression in gliomas. a Quantification of TGFβ2 mRNA expression levels in glioma patients in TCGA and CGGA datasets, and survival of patients with low/high coexpression of TAGLN2/TGFβ2. b Correlation between TAGLN2 and TGFβ2 in gliomas determined using TCGA (left panel) and CGGA (right panel) datasets. The statistical significance of correlation was evaluated using a linear regression model (TCGA all gliomacor = 0.634, P < 0.001; CGGA all gliomacor = 0.633, P < 0.001). c, d Western blot for TAGLN2 in cells treated with different concentrations of TGFβ2 (0, 1, 5 and 10 ng/mL) for 48 h. e Western blot for TAGLN2, N-cadherin, p-Smad2 and Smad2 in U87MG and U251 cells treated with TGFβ2 with/without SB431542 (5 and 10 μM). f Western blot for TAGLN2, p-Smad2 in U87MG and U251 cells transfected with Smad2 siRNA or controls. Results are representative of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001