Fig. 2

RBM38 upregulated the expression and the activity of PTEN in breast cancer cells. (a-d) BT474 and MDA-MB-453 cells were transfected with lentivirus containing either RBM38 overexpression or the control (NC). The expression of PTEN was increased after RBM38 upregulated both in protein (a, c) and mRNA levels (b, d). e-h BT474 and MDA-MB-453 cells were transfected with the knockdown RBM38 (sh1, sh2) and the control (SCR) lentivirus. PTEN expression was decreased after RBM38 downregulated both in protein (e, g) and mRNA levels (f, h). Western blot and qRT-PCR were performed to examine the expression of RBM38 and PTEN. i-l BT474 and MDA-MB-453 cells were transfected with lentivirus, containing RBM38 and control (NC), followed by PTEN immunoprecipitation (IP) and determination of its PIP3 phosphatase activity. The relative lipid phosphatase activity of PTEN was enhanced (i, k). Meanwhile, the PTEN lipid phosphatase activity in BT474 and MDA-MB-453 transfected with SCR, sh1and sh2 was inhibited (j, l). Data were means of three separate experiments and presented as mean ± SEM. Asterisk (*) indicates P-value <0.05. β-actin was used as protein loading control