Fig. 4

Multiple regions in the PTEN 3′-UTR were bound by RBM38 and responsive to RBM38. a Schematic representation of PTEN transcript and the location of probes used for REMSA. The AU-rich elements were shown in shaded boxes. b RBM38 bound to multiple regions in PTEN 3′-UTR, including probes B and D. RBM38 could not bind to probes a, c and e. c The schematic representation of the pGL3-PTEN 3′-UTR vector with various regions of PTEN 3′-UTR. d-e In BT474 (d) and MDA-MB-453 (e) cells, the relative luciferase activity of the reporter containing PTEN 3′-UTR B and D was increased by RBM38. The relative luciferase activity was calculated as a ratio of luciferase activity induced by RBM38 divided by that induced by an empty vector (mean ± SEM). * P < 0.05