Fig. 3

Effects of LATS2 on ESCC cell proliferation, colony formation, migration, and invasion in vitro. a QRT-PCR analysis of the relative expression of LATS2 in two groups of ESCC cells transfected with cDNA/pLATS2, siRNA/LATS2 and their corresponding control. b-c MTT and colony formation assays in ESCC cells after changed expressing of LATS2. d Wound scratch healing assay was performed to evaluate the motility of ESCC cells transfected with cDNA/pLATS2, siRNA/LATS2. Photographs were taken immediately (0 h) and at 48 h after wounding, quantification of wound closure was done. e Migration assay and Invasion assay were conducted to examine the invasion ability of ESCC cells. Results are expressed as the mean ± SD of three independent experiments. *: P < 0.05; **: P < 0.01