Fig. 4

Silencing of LATS2 reverses the effects of anti-miR-31 on phenotypes of ESCC cells. a Quantification of LATS2 expression was achieved by qRT-PCR transfected with anti-miR-31 or co-transfected with siRNA/LATS2. b-c MTT and colony formation assays were conducted to evaluate the proliferative capacity of ESCC cells. d-e. Wound healing assays were used to assess the motility of ESCC cells transfected with anti-miR-31 alone or co-transfected with siRNA/LATS2. f Migration and invasion assay were conducted in ESCC cells transfected with the same type. Results are expressed as the mean ± SD of three independent experiments. *: P < 0.05; **: P < 0.01