Fig. 1

The effects of calycosin on the proliferation of breast cancer cells and MCF10A cells. MCF-7, T47D, SKBR3, MDA-MB-468 and MCF10A cells were treated for 12, 24, or 48 h with calycosin (1–32 μM); then, cell proliferation was quantified using (a-c) the CCK-8 assay, (d) BrdU assay, and (e) colony formation assay. The results are from three independent experiments that were each conducted in triplicate. *p < 0.05 vs. control (0 μM)