Fig. 3

The expression levels of WDR7-7 and GPR30 in breast cancer cells and the association between WDR7-7 and GPR30. a The down-regulated expression of WDR7-7 was further confirmed in five breast cancer cell lines (MCF-7, T47D, SKBR3, MDA-MB-468, and MDA-MB-231). Representative data from three independent experiments are shown. *p < 0.05 vs. MCF10A cells. b-c The luciferase activity of MCF-7 and SKBR3 cells co-transfected with either a plasmid encoding wild-type or mutated 3′ UTR of GPR30 and either an empty vector or a plasmid encoding WDR7-7. Renilla luciferase activity was normalized to firefly luciferase activity and was plotted as relative luciferase activity. *p < 0.05 vs. vector. GPR30 protein expression levels in human breast cancer tissues (T) and adjacent normal tissues (N) were examined by (d) Western blot analyses with β-actin as the internal control; (e) HE staining and GPR30 immunohistochemistry (200× magnification)