Fig. 4

Effects of synthetic regulatory RNAs on luciferase activity, and MYC mRNA and protein levels. (a, b and c) When aRNA was not expressed in the three cell lines, the luciferase activity was significantly decreased between the NC iRNA group and iRNA group in T24, 5637 and HFF. However, the luciferase activity was still significantly decreased in HFF (a) after addition of aRNA in cells between these two groups. But the luciferase activity was no statistical difference in T24 (b) and 5637 (c). (d, e, f, g, h and i) Without aRNA, there was no difference between column 1 and 2 in all three cell lines and therefore the transcriptional and translational levels of MYC was not inhibited. After the cells were transfected with aRNA as shown in T24 and 5637, the mRNA and protein levels of MYC was largely reduced by expression of MYC amiRNA (column 4 in E, F, H and I) compared with the negative group (column 3 in e, f, h and i). However, there was no significant difference between column 3 and 4 (d and g) in HFF. All data are presented as the mean ± SD (*p < 0.05, **p < 0.01); bar, SD