Table 1 Relative sequences in this study
From: Synthetic regulatory RNAs selectively suppress the progression of bladder cancer
| Name | Relative sequence (5′-3′) |
|---|---|
| NC amiRNA |
CTCGAGAAGGTATATTGCTGTTGACAGTGAGCGCATTCTCCGAACGTGTCACGTATAGTG AAGCCACAGATGTATACGTGACACGTTCGGAGAATTTGCCTACTGCCTCGCTTCAAGGTA TATTGCTGTTGACAGTGAGCGCATTCTCCGAACGTGTCACGTATAGTGAAGCCACAGATG TATACGTGACACGTTCGGAGAATTTGCCTACTGCCTCGGCGGCCGC |
| MYC amiRNA |
CTCGAGAAGGTATATTGCTGTTGACAGTGAGCGCACAGAAATGTCCTGAGCAATATAGTG AAGCCACAGATGTATATTGCTCAGGACATTTCTGTTTGCCTACTGCCTCGCTTCCTTCCTTC AAGGTATATTGCTGTTGACAGTGAGCGCATGGACAGTGTCAGAGTCTATAGTGAAGCCAC AGATGTATAGACTCTGACACTGTCCATTTGCCTACTGCCTCGGCGGCCGC |
| iRNA | GAAUUCCGGAGGUCAGAACUCUUGG |
| aRNA |
CGCCAAGAGUUCUGUCCUCCGGUGGUGGUUAAUGAAAAUUAACUUACUAUACCAUAUA UCUCUAGA |
|
mutant hTERT promoter |
GGCCCCTCCCTCGGGTTACCCCACAGCCTAGGCCGATTCGACCTCTCTCCGCTGGGGCC CTCGCTGGCGTCCCTGCACCCTGGGAGCGCGAGCGGCGCGCGGGCGGGGAAGCGCGG CCCAGACCCCCGGGTCCGCCCGGAGCAGCTGCGCTGTCGGGGCCAGGCCGGGCTCCCA GTGGATTCGCGGGCACAGACGCCCAGGACCGCGCTCCCCACGTGGCGGAGGGACTGGG GACCCGGGCACCCGTCCTGCCCCTTCACCTTCCGGCTCCGCCTCCTCCGCGCGGACCCCG CCCCGTCCCGACCCCTTCCGGGTTTCCGGCCCAGCCCCTTCCGGGCCCTCCCAGCCCCTC CCCTTCCTTTCCGGGGCCCCGCCCTCTCCTCGCGGCGCGAGTTTCCGGCAGCGCTGCGTC CTGCTGCGCACGTGGGAAGCCCTGGCCC CGGCCACCCCCGCG |
| UAS | CGGAGTACTGTCCTCCG |
| GAL4-VP64 |
ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAG TGCT CCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCT ACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCA AGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATG ATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGA TAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTA ACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGT CAAAGACAGTTGACTGTATCGGGTTCCGGACGGGCTGACGCATTGGACGATTTTGATCTG GATATGCTGGGAAGTGACGCCCTCGATGATTTTGACCTTGACATGCTTGGTTCGGATGCCC TTGATGACTTTGACCTCGACATGCTCGGCAGTGACGCCCTTGATGATTTCGACCTGGACAT GCTGATTAAC |
|
si-MYC sense si-MYC antisense |
GCUUCACCAACAGGAACUATT UAGUUCCUGUUGGUGAAGCTT |