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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer

Fig. 2

Biological effects of EZH2 on PCa cell growth and colony forming in a miR-193a-dependent manner. a-c Western blot assays assessing the knocking down and overexpressing efficiency of si-EZH2, LV-shEZH2 and pcDNA3.1-EZH2 in PC3 and DU145 cell lines, GAPDH was used as an endogenous control. d–e The miR-193a expression was measured by qRT-PCR to verify the inhibitory efficiency of si-EZH2 + anti-miR-193a/si-EZH2 + anti-NC and overexpressing efficiency of pcDNA3.1-EZH2 + miR-193a/pcDNA3.1-EZH2 + miR-NC in both cell lines. U6 snRNA was measured as an internal control. CCK8 assays (f-i) and colony formation assays (j-l) evaluating the biological effects of EZH2 and miR-193a on the cell viability and proliferation in PCa cells. m-n GSEA showing that negatively enriched expression of gene sets was involved in hallmarks of mitotic spindle assembly and G2/M cell-cycle checkpoint in miR-193a-overexpressing PCa cells. Each bar represents the mean ± SD of three independent experiments. *P < 0.05

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