Fig. 5

Primary cells from GC tissue induced angiogenesis and metastasized in larval zebrafish (fli-eGFP). 600–800 primary cells for patient samples were fluorescently labeled in red and microinjected into yolk sac of each zebrafish embryo at 48 hpf (0 dpi). Primary cells from GC tissue induced angiogenesis at 1 dpi (a), and showed invasive behaviors at 1 dpi (b), 4 dpi (c) and 7 dpi (d) in zebrafish PDX model. At 7 dpi, we could detect cancer cells in the brain (e), and the caudal hematopoietic tissue (CHT) region (f). Arrow in (a) points to the tumor induced new blood vessels within tumor mass. Arrows in (c)-(f) point to the metastasized tumor cells in the head, trunk and tail of the zebrafish embryos. Hpf: hours post fertilization, dpi: days post injection