Fig. 4

Apigenin de-regulating STAT3 activation, similarly to NAC, reduces the expression of pro-survival molecules c-myc, cyclin D1, cFLIP and vFLIP. BC3 and BCBL1 were treated a with apigenin at 25 μM or b with NAC at 25 mM for 24 h and pSTAT3 (Tyr 705) and total STAT3 expression were assessed by western blotting. β-Actin was used as loading control. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of phosphorylated (p-STAT3) versus total protein (STAT3) and total protein (STAT3) protein versus β-Actin. c c-myc and cyclinD1 and d cFLIP and vFLIP expression was assessed by western blot analysis in PEL cells treated with apigenin at 25 μM for 24 h. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of specific proteins (c-myc, cyclin D1, cFLIP and vFLIP) versus β-Actin. Data are representative of at least three independent experiments